Automated DNA extraction:

ction is a key factor in molecular genetic testing. Automation of this process has some benefits, including increased throughput, more consistent and reproducible processing and improved sample tracking. Manufacturers have produced a plethora of platforms, with different extraction chemistries. However, the extraction of large blood volumes (>1 ml) is still a eparation Module I n), integrated with the Perkin Elmer Multiprobe II of paramagnetic e transferred from o-chemical qualities of the extracted dure (SOP) for the Therefore a study was performed, including over 180 anonymized whole blood samples on and the yield was as calculated as a ded in a multiplex directly loaded on was evaluated by mples, stored at extracted DNA for s assessed. tion platform is an resulting in DNA of good quality, with a high yield and a satisfying performance for diagnostic purposes. DNA extra important challenge, for which only a few systems are available. This EuroGentest project involves the assessment of the chemagic Magnetic S (chemagic MSM I) extraction robot (chemage (PE MPII) liquid handling robot. The extraction technology is based on the use beads with a DNA binding coating. Using magnetic rods, these beads can b one DNA washing buffer to another when applying an electro-magnetic field. The aim of this validation was to describe in detail the physic DNA. In addition, we wanted to compile a generic Standard Operating Proce use of this integrated system. EDTA. The DNA concentration was determined with a spectrophotometer determined in relation to the white blood cell count. The 260/280nm ratio w parameter for the quality of the DNA. In addition, all DNA samples were inclu drop-out PCR to check their initial performance. Furthermore, samples were agarose to detect possible early degradation of the DNA. Stability of the DNA repeating the multiplex PCR and the degradation test on randomly selected sa different conditions, during several months. Finally, the performance of the some in-house downstream diagnostic tests wa Our study has shown that the integrated chemagen-Perkin Elmer DNA extrac easy-to-use, flexible system